Abstract
Soils contaminated at different levels of crude oil were amended with two- particle-sized fractions of red mangrove biochar [ mm (B1) and 4 mm (B2)] from locally pyrolyzed red mangrove in earthen kiln and incorporated at five different rates (1, 2, 3, 4 and 6 mg ha-1) in the Teaching and Research Farm, Faculty of Agriculture, Niger Delta University, Bayelsa State, were assessed on the fate of bacteria population and its resultant effect on the soil organic matter, soil organic carbon, total nitrogen, and plant height. Total heterotrophic bacteria were ana- lyzed using the pour plate method with Nutrient Agar and selective nutrient me- dia such as Centramide Agar and Macconkey Agar for qualitative analysis, and the plates were incubated at 37oC for 24 hours. Twelve (12) bacteria were isolat- ed from the study, which is six Gram-stain-negative and six Gram-stain positive. The incorporation of B1 biochar was observed to influence the SOC significantly, SOM and TN reduced from 2.90 to 0.87 %, 5.00 to 1.50, and 0.13 to 0.4 % re- spectively while B2 recorded slight increases on same parameters. It is concluded that the application of locally pyrolyzed biochar for the amendment of crude oil contaminated soils had little influence on the bacteria population.
Keywords:
ed from the study
which is six gram-stain-negative and six gram-stain positive.
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